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HelpTest Code BILHA Schistosoma species Antibody, IgG, Serum
Useful For
Detection of antibodies to Schistosoma species
Reporting Name
Schistosoma Ab, IgG, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat inactivated | Reject |
Reference Values
Negative
Supportive Data
The Mayo Clinic Infectious Disease Serology laboratory evaluated the accuracy of the NovaTec Schistosoma mansoni IgG enzyme-linked immunosorbent assay (ELISA) (as performed in our laboratory) using 64 serum samples that were previously tested by a fluorescent microsphere immunoassay at Focus Diagnostics. A comparison of the results is shown in Table 1.
Table 1. Accuracy of the NovaTec Schistosoma IgG assay compared to the Focus (Quest) Diagnostics assay
|
n = 64 |
Focus (Quest) Diagnostics FMI |
||
|
Positive |
Negative |
||
|
NovaTec ELISA |
Positive |
31 |
1 |
|
Negative |
1 |
20 |
|
|
Equivocal |
5 |
6 |
|
Positive agreement (95% CI): 83.8% (68.5-92.6%)
Negative agreement (95% CI): 96.3% (80.2-100%)
Overall agreement (95% CI): 89.1% (82.6-97%)
The Mayo Clinic Infectious Disease Serology laboratory also evaluated the analytic specificity of the NovaTec S mansoni IgG ELISA using 36 serum samples positive for antibodies to other helminth and protozoa. The results are shown in Table 2 below.
Table 2. Analytical specificity studies
|
Specimen |
No. of specimens tested |
No. of sera positive or equivocal by the S mansoni IgG ELISA |
|
Entamoeba histolytica IgG Ab |
6 |
0 |
|
Echinococcus species IgG Ab |
6 |
3 |
|
Strongyloides ratti IgG Ab |
6 |
2 |
|
Taenia solium IgG Ab |
6 |
2 |
|
Trichinella spiralis IgG Ab |
6 |
3 |
|
Trypanosoma cruzi IgG Ab |
6 |
1 |
The reference range for the NovaTec S mansoni IgG ELISA was established by testing serum from 50 normal donors; 47/50 (94%) of healthy individuals were negative by this ELISA.
Day(s) Performed
Tuesday, Thursday
Report Available
Same day/1 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BILHA | Schistosoma Ab, IgG, S | 33317-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BILHA | Schistosoma Ab, IgG, S | 33317-9 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.