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HelpTest Code BILHA Schistosoma species Antibody, IgG, Serum
Useful For
Detection of antibodies to Schistosoma species
Reporting Name
Schistosoma Ab, IgG, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 30 days |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat inactivated | Reject |
Reference Values
Negative
Supportive Data
The Mayo Clinic Infectious Disease Serology laboratory evaluated the accuracy of the NovaTec Schistosoma mansoni IgG enzyme-linked immunosorbent assay (ELISA) (as performed in our laboratory) using 64 serum samples that were previously tested by a fluorescent microsphere immunoassay at Focus Diagnostics. A comparison of the results is shown in Table 1.
Table 1. Accuracy of the NovaTec Schistosoma IgG assay compared to the Focus (Quest) Diagnostics assay
|
n = 64 |
Focus (Quest) Diagnostics FMI |
||
|
Positive |
Negative |
||
|
NovaTec ELISA |
Positive |
31 |
1 |
|
Negative |
1 |
20 |
|
|
Equivocal |
5 |
6 |
|
Positive agreement (95% CI): 83.8% (68.5-92.6%)
Negative agreement (95% CI): 96.3% (80.2-100%)
Overall agreement (95% CI): 89.1% (82.6-97%)
The Mayo Clinic Infectious Disease Serology laboratory also evaluated the analytic specificity of the NovaTec S mansoni IgG ELISA using 36 serum samples positive for antibodies to other helminth and protozoa. The results are shown in Table 2 below.
Table 2. Analytical specificity studies
|
Specimen |
No. of specimens tested |
No. of sera positive or equivocal by the S mansoni IgG ELISA |
|
Entamoeba histolytica IgG Ab |
6 |
0 |
|
Echinococcus species IgG Ab |
6 |
3 |
|
Strongyloides ratti IgG Ab |
6 |
2 |
|
Taenia solium IgG Ab |
6 |
2 |
|
Trichinella spiralis IgG Ab |
6 |
3 |
|
Trypanosoma cruzi IgG Ab |
6 |
1 |
The reference range for the NovaTec S mansoni IgG ELISA was established by testing serum from 50 normal donors; 47/50 (94%) of healthy individuals were negative by this ELISA.
Method Description
The qualitative immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the enzyme-linked immunosorbent assay (ELISA) technique.
Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material, horseradish peroxidase-labelled protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product.
The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader.(Package insert: NovaLisa Schistosoma mansoni, Gold Standard Diagnostics; V SCHG0410engl,dt,fr,it,es18082009-JH Rev 01)
Day(s) Performed
Tuesday, Thursday
Report Available
Same day/1 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BILHA | Schistosoma Ab, IgG, S | 33317-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BILHA | Schistosoma Ab, IgG, S | 33317-9 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: