Home
HelpTest Code lab1231754 Tropheryma whipplei, Molecular Detection, PCR, Blood
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Tropheryma whipplei PCR, BSpecimen Type
Whole Blood EDTASpecimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Tropheryma whipplei DNA is unlikely.
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube (preferred)
Reject Due To
| Bone marrow | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days |
| Ambient | 7 days | |
| Frozen | 7 days |
Specimen Minimum Volume
0.5 mL
Special Instructions
Day(s) Performed
Monday through Sunday
Specimen Retention Time
7 daysReport Available
2 to 7 daysReference Values
Not applicable
Useful For
Aiding in the diagnosis of Whipple disease, especially for identifying inconclusive or suspicious cases, using whole blood specimens
Testing Algorithm
For information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.
CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| WHIPB | Tropheryma whipplei PCR, B | 97205-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC89 | Specimen Source | 31208-2 |
| 56064 | Tropheryma whipplei PCR, B, Result | 97205-9 |
Method Description
Nucleic acid is extracted from all specimens using the MagNA Pure extraction system. The resulting nucleic acid is tested for the presence of the target DNA of Tropheryma whipplei using the LightCycler real-time polymerase chain reaction (PCR). The instrument amplifies and continuously monitors the development of target nucleic acid using fluorescent resonance emission technology after each cycle. Analysis of the PCR amplification and probe melting curves is accomplished through the use of the LightCycler software.(Sloan LM, Rosenblatt JE, Cockerill FR III. Detection of Tropheryma whipplei DNA in clinical specimens by LightCycler real-time PCR. J Clin Microbiol. 2005;43[7]:3516-3518; Geibdorfer W, Moter A, Bogdan C. Tropheryma whipplei, In: Carroll K, Pfaller M, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:1189-1197)
Supportive Data
A total of 321 clinical specimens (including blood, tissue, cerebrospinal fluid, and synovial fluid) were evaluated for the presence of Tropheryma whipplei DNA by targeting the heat shock protein 65 gene using the LightCycler Whip assay and results were compared to those of a conventional polymerase chain reaction (PCR) assay. The sensitivity and specificity of the LightCycler Whip assay compared to conventional PCR were 98% and 99%, respectively. The analytical sensitivity was less than 50 targets per reaction. The LightCycler Whip showed no cross reaction when tested on a panel of 28 organisms genotypically closely related to T whipplei by BLAST analysis.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Microbiology Test Request (T244)