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Test Code RPRT3 Rapid Plasma Reagin (RPR) with Reflex to Treponema pallidum Particle Agglutination, Serum


Ordering Guidance


This assay should only be used following an initial reactive treponemal antibody assay result as part of the reverse syphilis testing algorithm.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.3 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Diagnosing syphilis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RPRT4 RPR, Titer, S No No
RTPPA Syphilis Ab, TP-PA, S Yes, (Order TPPA) No

Testing Algorithm

If this test is positive, then the rapid plasma reagin titer will be performed at an additional charge.

 

If this test is negative, then the Treponema pallidum particle agglutination test will be performed at an additional charge.

 

For more information see Syphilis Serology Algorithm.

Special Instructions

Reporting Name

RPR w/ Reflex to TP-PA, S

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Negative

 

Reference values apply to all ages.

Method Description

If the rapid plasma reagin (RPR) screen is reactive, the RPR titer is performed. The RPR titer test is a macroscopic screening assay done with unheated serum. Reagin reacts with nontreponemal antigen containing colloidal charcoal particles. This reaction results in a visual flocculation of the black particles against the white card background. The test yields a positive or negative result, and all positive samples are titered to determine the highest positive dilution.(Huber TW, Storms S, Young P, et al. Reactivity of microhemagglutination, fluorescent treponemal antibody absorption, Venereal Disease Research Laboratory, and rapid plasma reagin tests in primary syphilis. J Clin Microbiol. 1983;17[3]:405-409; Kaur G, Kaur P. Syphilis testing in blood donors: an update. Blood Transfus. 2015;13[2]:197-204)

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 4 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86592

86593- (if appropriate)

86780- (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RPRT3 RPR w/ Reflex to TP-PA, S 20507-0

 

Result ID Test Result Name Result LOINC Value
616970 RPR w/ Reflex to TP-PA, S 20507-0

Forms

If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.