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HelpTest Code PLFAT Cryptococcus Antigen Titer, Lateral Flow Assay, Pleural Fluid (Non-Orderable)
Specimen Required
Only orderable as a reflex. For more information see PLFA / Cryptococcus Antigen Screen, Lateral Flow Assay, Pleural Fluid.
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Collect pleural fluid in a sterile vial.
Useful For
Only orderable as a reflex. For more information see PLFA / Cryptococcus Antigen Screen, Lateral Flow Assay, Pleural Fluid.
Diagnosis of infection with Cryptococcus species
Reporting Name
Cryptococcus Ag Titer, LFA, PFSpecimen Type
Pleural FluidSpecimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Pleural Fluid | Refrigerated (preferred) | 21 days |
| Frozen | 30 days |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
Only orderable as a reflex. For more information see PLFA / Cryptococcus Antigen Screen, Lateral Flow Assay, Pleural Fluid.
Negative
Method Description
The CrAg (Cryptococcus antigen) lateral flow assay is a sandwich immunochromatographic assay. Specimens and diluent are added to a test tube and the lateral flow device is added. The test uses specimen wicking to capture gold-conjugated anti-cryptococcal antigen monoclonal antibodies and gold-conjugated control antibodies deposited on the test membrane. If cryptococcal antigen is present in the specimen, it binds to the gold-conjugated, anti-cryptococcal antigen antibodies. This complex wicks up the membrane and interacts with the test line, which has immobilized anti-cryptococcal antigen monoclonal antibodies. The antigen-antibody complex forms a sandwich at the test line causing a visible line to form. A valid test shows a visible line at the control line. Positive test results create 2 lines (control and specimen), while negative results form only the control line.(Package insert: CrAg Lateral Flow Assay. IMMY; Rev 06/27/2019)
Day(s) Performed
Monday through Sunday
Report Available
2 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87899
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PLFAT | Cryptococcus Ag Titer, LFA, PF | 11473-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 48431 | Cryptococcus Ag Titer, LFA, PF | 11473-6 |
Disease States
- Cryptococcosis