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HelpTest Code P5NT Pyrimidine 5' Nucleotidase, Blood
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Pyrimidine 5' Nucleotidase, BSpecimen Type
Whole Blood ACD-BSpecimen Required
Specimen Type: Whole blood
Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: Lavender top (EDTA)
Specimen Volume: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Reject Due To
| Gross hemolysis | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD-B | Refrigerated | 20 days |
Specimen Minimum Volume
3 mL
Day(s) Performed
Monday through Friday
Specimen Retention Time
7 daysReport Available
10 daysReference Values
Normal
Useful For
Evaluation of marked basophilic stippling
Evaluation of hemolytic anemia
CPT Code Information
83915
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| P5NT | Pyrimidine 5' Nucleotidase, B | 2902-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 2734 | Pyrimidine 5' Nucleotidase, B | 2902-5 |
Method Description
Pyrimidine nucleotides have a spectral absorption curve that is markedly different from that exhibited by (normally present) adenine nucleotides (eg, adenosine triphosphate). The former have a peak at about 270 nm; the latter at about 257 nm. Thus, pyrimidine 5' nucleotidase deficiency may be ascertained by demonstrating a very high spectral absorption maximum of 270 nm in erythrocyte extracts.(Beutler E. Red Cell Metabolism. A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:100-102; van Solinge WW, van Wijk. Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.