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Test Code P40NA p40 + Napsin A Immunostain, Technical Component Only


Advisory Information


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html


Useful For

p40 aids in the classification of carcinomas and lymphomas

 

Napsin A aids in the identification of primary lung adenocarcinoma

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHMTO IHC Multiplex, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

p40 + Napsin A IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

p40 is an antibody (detected by the chromogen 3,3'-diaminobenzidine [DAB]) that recognizes the deltaNp63 isoform of p63. This isoform may exert an oncogenic effect and is selectively expressed in squamous cell carcinoma. Napsin A is an aspartic proteinase involved in the proteolytic processing of surfactant precursors in the normal alveolar epithelium. In normal tissues, napsin A is expressed in the cytoplasm of alveolar macrophages, type II pneumocytes, pancreatic ducts and acini, and in renal tubules (detected by the chromogen fast red). Napsin A has clinical utility for the identification of primary lung adenocarcinomas. Napsin A is also positive in a subset of thyroid and renal cell carcinomas (especially papillary types).

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions

No significant cautionary statements.

Clinical Reference

1. Whithaus K, Fukuoka J, Prihoda TJ, Jagirdar J: Evaluation of napsin A, cytokeratin 5/6, p63, and thyroid transcription factor 1 in adenocarcinoma versus squamous cell carcinoma of the lung. Arch Pathol Lab Med 2012 Feb;136(2):155-162

2. Sterlacci W, Savic S, Schmid T, et al: Tissue-Sparing Application of the Newly Proposed IASLC/ATS/ERS Classification of Adenocarcinoma of the Lung Shows Practical Diagnostic and Prognostic Impact. Am J Clin Pathol 2012;137:946-956

3. Nonaka D: A study of deltaNp63 expression in lung non-small cell carcinomas. Am J Surg Pathol 2012;36(6):895-899

4. Kargi A, Gurel D, Tuna B: The diagnostic value of TTF-1, CK 5/6, and p63 immunostaining in classification of lung carcinomas. Appl Immunohistochem Mol Morphol 2007 Dec;15(4):415-420

Method Description

Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Specimen Retention Time

Until staining is complete.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88344-TC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
P40NA p40 + Napsin A IHC, Tech Only In Process

 

Result ID Test Result Name Result LOINC Value
70838 p40 + Napsin A IHC, Tech Only No LOINC Needed