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HelpTest Code NMH24 N-Methylhistamine, 24 Hour, Urine
Ordering Guidance
Random urine collections are preferred for patients with episodic symptoms, eg, in the context of allergic reactions, brought on by specific environmental factors; order NMHR / N-Methylhistamine, Random, Urine.
If the total volume provided is less than 300 mL, this test will be canceled and NMHR ordered and performed.
Necessary Information
Specimen volume (in milliliters) and duration are required.
Specimen Required
Patient Preparation: Patients taking monoamine oxidase inhibitors (MAOIs) may have increased levels of N-methylhistamine. If medically feasible, for 2 weeks before specimen collection, patient should not take MAOIs.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plastic, 5-mL tube
Specimen Volume: 5 mL
Collection Instructions:
1. Collect urine for 24 hours.
2. No preservative.
3. Aliquot into plastic tube and send at refrigerate temperature.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Useful For
Screening for and monitoring of mastocytosis and disorders of systemic mast-cell activation, such as anaphylaxis and other forms of severe systemic allergic reactions using 24-hour urine collection specimens
Monitoring therapeutic progress in conditions that are associated with secondary, localized, low-grade persistent, mast-cell proliferation and activation such as interstitial cystitis
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| NMH1D | N-Methylhistamine, 24 Hr, U | No | Yes |
| CRT24 | Creatinine, 24 HR, U | No | Yes |
Special Instructions
Reporting Name
N-Methylhistamine, 24 Hr, USpecimen Type
UrineSpecimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 14 days |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
N-METHYLHISTAMINE
0-5 years: 85-369 mcg/g creatinine
6-16 years: 48-238 mcg/g creatinine
>16 years: 19-143 mcg/g creatinine
CREATININE
Males: 930-2955 mg/24 hours
Females: 603-1783 mg/24 hours
Reference values have not been established for patients who are younger than 18 years.
Method Description
N-methylhistamine:
Isotopically labeled N-methylhistamine (NMH) is added to the sample as an internal standard (IS). NMH and IS are then purified from urine using solid phase extraction. The eluate is analyzed using liquid chromatography-tandem mass spectrometry. (Unpublished Mayo method)
Creatinine:
The enzymatic method is based on the determination of sarcosine from creatinine with the aid of creatininase, creatinase, and sarcosine oxidase. The liberated hydrogen peroxide is measured via a modified Trinder reaction using a colorimetric indicator. Optimization of the buffer system and the colorimetric indicator enables the creatinine concentration to be quantified both precisely and specifically.(Package insert: Creatinine plus ver 2. Roche Diagnostics; V15.0, 03/2019)
Day(s) Performed
Monday, Wednesday, Friday
Report Available
3 to 7 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| NMH24 | N-Methylhistamine, 24 Hr, U | 44340-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CR_A | Creatinine, 24 HR, U | 2162-6 |
| 605159 | N-Methylhistamine, 24 Hr | 44340-8 |
| TM27 | Collection Duration (h) | 13362-9 |
| VL69 | Urine Volume (mL) | 3167-4 |
| CR_24 | Creatinine Concentration, 24 HR, U | 20624-3 |