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HelpTest Code MTBT Mycobacterium tuberculosis Complex, Molecular Detection, PCR, Paraffin, Tissue
Useful For
Preferred method for rapid detection of Mycobacterium tuberculosis complex DNA in formalin-fixed, paraffin-embedded tissue specimens
Detecting M tuberculosis complex
This test is not intended for the detection of latent tuberculosis and must not be used as a substitute for tests intended for detection of latent tuberculosis such as the tuberculin skin test or an interferon gamma release assay.
Reporting Name
MTB complex PCR, FFPESpecimen Type
Tissue, ParaffinAdditional Testing Requirements
When non-fixed specimen is available, a mycobacterial culture on that specimen must always be performed in addition to this test. If your facility is unable to perform mycobacterial culture, order CTB / Mycobacteria and Nocardia Culture, Varies concurrently with this test.
Necessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycobacterium tuberculosis DNA is unlikely.
Preferred Paraffin-embedded tissue block:
Supplies: Tissue Block Container (T553)
Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)
Sources: Body tissue
Container/Tube: Tissue block
Collection Instructions: Submit a FFPE tissue block to be cut and returned.
Acceptable Paraffin-embedded tissue block:
Specimen Type: Formalin-fixed, paraffin-embedded tissue block (FFPE)
Sources: Body tissue
Container/Tube: Sterile container for each individual cut section (scroll).
Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.
Specimen Minimum Volume
See Specimen Required.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue, Paraffin | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Any non-formalin-fixed, paraffin-embedded tissue block(FFPE) FFPE slides FFPE body fluids |
 Reject |
Reference Values
Not applicable
Day(s) Performed
Monday through Sunday
Report Available
5 to 7 daysSpecimen Retention Time
7 days; after which time the block will be returned to the clientPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87556
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MTBT | MTB complex PCR, FFPE | 38379-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRCTB | MTB Complex PCR, FFPE, Source | 31208-2 |
| TBRR | MTB Complex PCR, FFPE, Result | 38379-4 |