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HelpTest Code LAB986 Free Fatty Acids, Total, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Free Fatty Acids, Total, SSpecimen Type
SerumSpecimen Required
Patient Preparation:
1. Fasting: 12 hours, required
2. Patient must not consume any alcohol for 24 hours before the specimen is collected.
3. Patient should not be receiving therapeutic heparin.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Within 45 minutes of collection, centrifuge and aliquot 1 mL of serum into a plastic vial.
2. Immediately freeze specimen.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen | 7 days |
Specimen Minimum Volume
0.5 mL
Day(s) Performed
Monday through Friday
Specimen Retention Time
7 daysReport Available
1 to 3 daysReference Values
≥18 years: 0.00-0.72 mmol/L
Reference values have not been established for patients who are <18 years of age.
Useful For
Evaluation of metabolic status of patients with endocrinopathies
Monitoring of control of diabetes mellitus
Monitoring the effects of therapeutic diet/exercise lifestyle changes
CPT Code Information
82725
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| NEFA | Free Fatty Acids, Total, S | 15066-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| NEFA | Free Fatty Acids, Total, S | 15066-4 |
Method Description
In the presence of adenosine triphosphate and coenzyme A (CoA), serum non-esterified fatty acids form acyl-CoA, adenosine monophosphate and pyrophosphate when treated with acylCoA synthetase. The acylCoA is then oxidized by adding acylCoA oxidase to produce hydrogen peroxide, which in the presence of added peroxidase allows for the oxidative condensation of 3-methyl-N- ethyl-N-(beta-hydroxyethyl)-aniline with 4-aminoantipyrine to form a purple colored end product.(Package insert: HR Series NEFA-HR(2). Fujifilm Healthcare Americas Corporation; 22.01.20K08 12/2021)
Forms
If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.