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HelpTest Code LAB877 AFB Culture and Smear
Useful For
Use: To culture tissue or body fluids in suspected cases of Mycobacterial disease.
Reflex Testing: May include: DNA Probe; further ID and sensitivities must be requested by ordering physician.
Performing Laboratory
Asante Rogue Regional Medical Center
Performing Department
Microbiology
Specimen Required
For collection of sputa, the patient is instructed to cough deeply into a sterile container. At least 5 mL of sputum per specimen is requested. An early morning sputum is the specimen of choice. For best recovery of organisms, 3 separate specimens collected over 3 consecutive days are submitted.
For sputum, the patient should vigorously rinse his/her mouth with sterile water 3 times prior to collection. Dentures should be removed. Oral antiseptics must be avoided prior to specimen collection.
Collect in sterile container.
Specimen Minimum Volume
See attached chart as varies based upon specimen type
Specimen Stability Information
See attached chart as varies based upon specimen type
Day(s) Performed
Monday through Sunday
Report Available
- Turnaround time for smear is 24 hours.
- Preliminary report: weekly (beginning at 2 weeks); final report: 8 weeks.
- If reflex testing is required, test results will be delayed.
Method Name
Culture on Selective Media and Continuous Monitoring Mycobacterial Culture System
CPT Code Information
87206 Stain, AFB – Smear, primary source with interpretation
87015 AFB Concentration – (any type), for infectious agents
87116 Culture, Mycobacteria - or other acid-fast bacilli