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Test Code LAB857 Intrinsic Factor Blocking Antibody, Serum

Important Note

Mayo's test code: IFBA

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Intrinsic Factor Blocking Ab, S

Specimen Type

Serum


Ordering Guidance


For a comprehensive workup of patients with suspected pernicious anemia, order ACASM / Pernicious Anemia Cascade, Serum, which initiates testing with measurement of vitamin B12. Depending of the vitamin B12 concentration, testing for intrinsic factor blocking antibody, gastrin, and methylmalonic acid may be added.



Specimen Required


Patient Preparation:

1. Fasting: 8 hours, required

2. This test should not be performed on patients who have received a vitamin B12 injection or radiolabeled vitamin B12 injection within the previous 2 weeks.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Specimen Minimum Volume

Serum: 0.5 mL

Special Instructions

Day(s) Performed

Monday through Friday

Specimen Retention Time

2 weeks

Report Available

1 day to 3 days

Reference Values

Negative

Useful For

Confirming the diagnosis of pernicious anemia

Testing Algorithm

For information see Vitamin B12 Deficiency Evaluation.

CPT Code Information

86340

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IFBA Intrinsic Factor Blocking Ab, S 31444-3

 

Result ID Test Result Name Result LOINC Value
IFBLA Intrinsic Factor Blocking Ab, S 31444-3
CMT31 Comment 48767-8

Method Description

The Access Intrinsic Factor (IF) Antibody assay is a competitive binding immunoenzymatic assay. The sample is added to a reaction vessel along with IF alkaline phosphatase conjugate and a protein blocking solution. IF antibody in the sample binds to the IF conjugate. After incubation in a reaction vessel, paramagnetic particles coated with a mouse monoclonal antibody, specific for the vitamin B12 binding site on IF, is added to the reaction. IF conjugate that has not been blocked by sample anti-IF binds to the monoclonal antibody on the solid phase. After an additional incubation, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Chemiluminescent substrate is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is inversely proportional to the concentration of IF antibody in the sample expressed in AU/mL (antibody units/mL). The amount of analyte in the sample is determined from a stored calibration.(Package insert: Access Intrinsic Factor Ab. Beckman Coulter, Inc; 06/2020)