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HelpTest Code LAB770 Jo 1 Antibodies, IgG, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Jo 1 Ab, IgG, SSpecimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-Treated | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Specimen Minimum Volume
0.35 mL
Special Instructions
Day(s) Performed
Monday through Saturday
Specimen Retention Time
14 daysReport Available
1 to 3 daysReference Values
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Useful For
Evaluating patients with clinical features of idiopathic inflammatory myositis, especially those with clinical features suggestive of anti-synthetase syndrome or interstitial lung disease
Testing Algorithm
For more information see Connective Tissue Disease Cascade.
CPT Code Information
86235
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| JO1 | Jo 1 Ab, IgG, S | 33571-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| JO1 | Jo 1 Ab, IgG, S | 33571-1 |
Method Description
Recombinant Jo 1 antigen is coupled covalently to polystyrene microspheres, which are impregnated with fluorescent dyes to create a unique fluorescent signature. Jo 1 antibodies, if present in diluted serum, bind to the Jo 1 antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated antihuman IgG antibody is then added to detect IgG anti-Jo 1 bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens, and a secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for Jo 1 microspheres to a 4-point calibration curve.(Package insert: Bioplex 2200 ANA Screen. Bio-Rad Laboratories, 02/2019)
Forms
If not ordering electronically, complete, print, and send a Kidney Transplant Test Request with the specimen.