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Test Code LAB727 Glomerular Basement Membrane Antibodies, IgG, Serum

Important Note

Mayo code is GBM

ASANTE order code: AGLOM

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Glomerular Basement Membrane IgG Ab

Specimen Type

Serum


Ordering Guidance


If patient is being evaluated for autoimmune skin disease, order CIFS / Cutaneous Immunofluorescence Antibodies (IgG), Serum for evaluation of anti-intercellular substance (ICS) and antibasement membrane zone (BMZ) antibodies.



Specimen Required


Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Specimen Minimum Volume

0.35 mL

Day(s) Performed

Monday through Friday

Specimen Retention Time

14 days

Report Available

2 to 3 days

Reference Values

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Useful For

Evaluating patients with clinical features of anti-glomerular basement disease, including rapidly progressive glomerulonephritis or pulmonary hemorrhage

CPT Code Information

83516

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GBM Glomerular Basement Membrane IgG Ab 31254-6

 

Result ID Test Result Name Result LOINC Value
GBM Glomerular Basement Membrane IgG Ab 31254-6

Method Description

Glomerular basement membrane (GBM) antigen is covalently coupled to polystyrene microspheres that are impregnated with fluorescent dyes to create a unique fluorescent signature. GBM antibodies, if present in diluted serum, bind to the GBM antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated antihuman-IgG antibody is then added to detect IgG anti-GBM bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens. A secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for GBM microspheres to a 4-point calibration curve.(Package insert: Bio-Plex 2200 Vasculitis. Bio-Rad Laboratories; 12/2018)

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.