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Test Code LAB61 Cortisol

Performing Laboratory

Asante Rogue Regional Medical Center

Specimen Minimum Volume

0.5 mL

Billing Code

2070100

Methodology

Chemiluminescence (CLIA)

Specimen Requirements

Yellow (Gold) top tube (SST)

Lithium Heparin Green top tube

 

Outpatient clients MUST draw SST

Blood may be drawn between:

5:00 and 10:00 A.M. for A.M. draw

After 4:00 P.M. for P.M. draw.

Performing Department

Chemistry

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

82533  -  Cortisol; total

Additional Information

Used to establish a diagnosis of adrenocortical insufficiency, Addison’s disease, adrenocortical hypersecretion, Cushing’s disease; malfunction of any of the organs in the hypothalamus, pituitary, adrenal cortex axis will result in alteration of the cortisol levels.  Cortisol is physiologically increased in patients with hypoglycemia, stress, and in pregnancy.

Cortisol Stimulation Test:

(1) Baseline Cortisol
(2) Inject Cortrosyn or equivalent
(3) 30 minute post-injection Cortisol
(4) 60 minute post-injection Cortisol

Cortisol levels usually peak about 45 to 60 minutes after injection of Cortrosyn.  The normal response is an approximate doubling of the cortisol value.

Specimen Transport Temperature and Stability

Centrifuge and refrigerate within 2 hours of collection

Primary tube: Spun SST stable 24 hours at 2° to 8° C

Serum stable 48 hours if aliquoted and refrigerated at 2° to 8° C

Reasons for Rejection

Hemolysis

Quantity not sufficient (QNS)

Lack of Two Patient Identifiers:

         1-Patient's First & Last name 

         2-Patient's Date of Birth