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HelpTest Code LAB536 1,25-Dihydroxyvitamin D, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
1,25-Dihydroxyvitamin D, SSpecimen Type
SerumOrdering Guidance
The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum is the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) may be needed to adequately assess vitamin D status.
Specimen Required
Patient Preparation:
Fasting: 4 hours, preferred but not required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 30 days |
| Frozen | 30 days | |
| Ambient | 7 days |
Specimen Minimum Volume
Serum: 0.7 mL
Day(s) Performed
Monday through Friday
Specimen Retention Time
2 weeksReport Available
2 to 5 daysReference Values
Males:
<16 years: 24-86 pg/mL
≥16 years: 18-64 pg/mL
Females:
<16 years: 24-86 pg/mL
≥16 years: 18-78 pg/mL
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Useful For
Evaluation of selected patients with disorders of calcium and phosphate metabolism
Evaluation of chronic kidney disease with suspected abnormalities of vitamin D metabolism
Investigation of suspected hereditary disorders of vitamin D metabolism, including vitamin D-dependent rickets
Evaluation of parathyroid hormone-independent hypercalcemia, particularly when granulomatous disease, lymphoma, or CYP24A1 deficiency is suspected
As a second-line test following measurement of 25-hydroxyvitamin D when clinically indicated
CPT Code Information
82652
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DHVD | 1,25-Dihydroxyvitamin D, S | 62290-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8822 | 1,25-Dihydroxyvitamin D, S | 62290-2 |
Method Description
Deuterated isotopically labeled 1,25-dihydroxyvitamin D2 and D3 are added to the sample as internal standards. 1,25-Dihydroxyvitamin D2 and D3 and the internal standards are extracted using immunopurification. Extracts are then derivatized and analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Renal Diagnostics Test Request (T830)