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Test Code LAB5251 GBS (Group B Beta Strep) by PCR (Nucleic Acid Amplification)

Useful For

Intrapartum screening assay for detection of presumptive GBS colonization.

Special Instructions

Intrapartum testing can only be performed on patients 14 years of age and older.  For patients <14 years old and/or antepartum, Group B Strep Culture (LAB1377) should be performed.

Performing Laboratory

Asante Rogue Regional Medical Center

 

Performing Department

Microbiology

Specimen Required

Swabbed sample from the vagina and rectum of obstetric patients.
 

ONLY Red-capped BBL Liquid Stuart dual swab culturettes are acceptable for this test.

BBL Culturette II Swab Red Cap

 

Using proper hand hygiene and PPE per standard precautions, collect the specimen aseptically as follows:


Follow patient identification and specimen labeling protocols.

     1. Wipe away excessive amounts of secretion or discharge.

     2. Remove both marked swabs from the transport container.

     3. Carefully insert both marked swabs into the patient’s vagina. Sample secretions from the mucosa of the  

         lower one-third part of the vagina. Rotate the swabs three times to ensure uniform sample on both swabs.

     4. Using the same marked swabs, carefully insert both swabs approximately 2.5 cm beyond the anal

         sphincter, and gently rotate to sample anal crypts.

     5. Place both marked swabs in the transport container.

Specimen Stability

Specimens can be stored up to 24 hours at 2-25 °C

Reject Due To

  • Patient <14 years old
  • Swab collected >24 hours prior
  • Specimen collected using device other than BBL Culturette II swabs

Interpretation

  • GBS Positive- GBS target DNA is detected – Patient likely colonized with GBS.
  • GBS NEGATIVE- GBS target DNA cannot be detected - Patient may/may not be colonized with GBS.
  • GBS INVALID -Presence or absence of the GBS target DNA cannot be determined. Possible interfering substance present. Recollect if clinically indicated.  

Method Name

Cepheid Xpert System

Day(s) Performed

Monday through Sunday

On demand 24/7.

Target: 2 hours from receipt in laboratory

CPT Code Information

87653 – Infectious agent detection by nucleic acid (DNA or RNA)

Billing Code

2071769