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HelpTest Code LAB52 Direct Bilirubin
Performing Laboratory
Asante Rogue Regional Medical Center / Asante Three Rivers Medical Center / Asante Ashland Community Hospital / Asante White City Laboratory
Specimen Minimum Volume
0.5 ml
Billing Code
2050144
Methodology
Diazo Reaction
Specimen Requirements
Lithium Heparin Green top tube
Infants Only: 1 full Green or Yellow microtainer
Reasons for Rejection
Newborns only: Microtainer samples should be protected from light using either an amber colored microtainer or other light protection means. If unprotected, microtainers are only stable for 1 hour from time of draw.
Hemolysis
Quantity not sufficient (QNS)
Lack of Two Patient Identifiers:
1-Patient's First & Last name
2-Patient's Date of Birth
Day(s) Test Set Up
Monday through Sunday
Routine – same day
ASAP – 2 hours after receipt of specimen in lab
STAT – 1 hour after receipt of specimen in lab
Test Classification and CPT Coding
82248 - Bilirubin; direct
Additional Information
Yellow bile pigment is produced normally as a result of
breakdown of aging red blood cell hemoglobin. Bilirubin is
composed of two components – conjugated
(“direct”) and unconjugated. Increased direct
bilirubin occurs with biliary diseases, including both intrahepatic
and extrahepatic lesions. Hepatocellular causes of elevation
include hepatitis, cirrhosis and advanced neoplastic
states. Increased with cholestatic drug reactions,
Dubin-Johnson Syndrome and Rotor Syndrome.
LIMITATIONS: Placental cord blood samples may yield elevated values. Visibly hemolyzed samples may yield spurious results.
CONTRAINDICATIONS: Usually not necessary when the
total bilirubin is normal.
Specimen Transport Temperature and Stability
Protect from light; Exposure will decrease results
Centrifuge and refrigerate within 2 hours of collection
When well protected from light, bilirubin in serum or plasma
is stable for 3 days at 2° to 8° C
Performing Department
Chemistry