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Test Code LAB5171 Immunofixation, Serum

Important Note

Serum Immunofixation alone does not adequately screen for monoclonal gammopothies.

If indicated order Monoclonal Protein Panel:

Asante Order Code: MPP Epic Beaker Code: LAB1232522

This will include both serum protein electrophoresis and serum free light chains.

Serum Immunofixation can be added if requested.

Useful For

Use: Primary methodology used for Immunotyping is the MiniCap capillary electrophoresis. Charged molecules are separated by their electrophoretic mobility in an alkaline buffer with a specific pH in tubes with internal diameter of 100 um. Separation also occurs according to the electrolyte pH and electroosmotic flow.

For additional information, the immunofixation by gel ELP may be used.The immunofixation electrophoresis method is a combination of two techniques, gel electrophoresis followed by immunofixation, where an antigen is fixed” in a gel by an antibody through the formation of an insoluble immune complex.  IFE has become a useful clinical tool for examination and identification of various serum protein abnormalities, primarily monoclonal ammopathies.

 

Test includes typing of monoclonal proteins for light chain specificity.

Performing Laboratory

Asante Rogue Regional Medical Center (ARRMC)

 

Performing Department

Special Chemistry

Specimen Required

Plain Red top tube

Yellow (Gold) top tube (SST)

Minimum volume is 1.0 ml

Specimen Minimum Volume

1.0 mL

Specimen Stability Information

Centrifuge collection tube within two hours of draw. 
Refrigerate serum up to 7 days. Freeze up to 3 months.

Reject Due To

Hemolysis

Lipemia

Plasma

Day(s) Performed

Tests are batched and performed daily Monday through Friday, A.M. shift. 

Method Name

Primary method is Sebia Capillarys Immunotyping.

CPT Code Information

86320  Immunoelectrophoresis; serum

Billing Code

2070357

Secondary ID

Immunoelectrophoresis