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Test Code LAB123781 PML::RARA Quantitative, PCR, Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

PML/RARA Quantitative, PCR

Specimen Type

Varies


Ordering Guidance


This assay may not detect rare, unusual PML::RARA fusions. Therefore, if the assay is going to be used for monitoring after treatment, the test should be performed at the time of diagnosis to ensure that the test gives a positive result.



Shipping Instructions


1. Refrigerated specimens must arrive within 5 days of collection, and ambient specimens must arrive within 3 days of collection.

2. Collect and package specimen as close to shipping time as possible.



Necessary Information


The following information is required:

1. Pertinent clinical history

2. Date of collection

3. Specimen source (blood or bone marrow)



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Whole blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot

3. Label specimen as bone marrow.


Reject Due To

Gross hemolysis Reject
Moderately to severely clotted Reject

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 5 days PURPLE OR PINK TOP/EDTA
  Ambient  72 hours PURPLE OR PINK TOP/EDTA

Specimen Minimum Volume

Blood: 8 mL; Bone Marrow: 2 mL

Day(s) Performed

Monday through Saturday

Specimen Retention Time

Blood/Bone marrow: 2 weeks; Extracted RNA: 3 months

Report Available

4 to 8 days

Reference Values

An interpretive report will be provided.

 

If positive, a value representing a ratio of PML::RARA fusion transcript to the control gene ABL1 expressed as a percentage will be reported.

Useful For

Diagnosis of acute promyelocytic leukemia (APL)

 

Detection of residual or recurrent APL

 

Monitoring the level of PML::RARA (promyelocytic leukemia/retinoic acid receptor alpha) in APL patients

Testing Algorithm

For more information see Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up

CPT Code Information

81315-PML/RAR-alpha (t(15;17)), (PML-RARA regulated adaptor molecule 1) (eg, promyelocytic leukemia) translocation analysis; all breakpoints (eg, intron 3, intron 6 and variable in exon 6), qualitative or quantitative

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PMLR PML/RARA Quantitative, PCR In Process

 

Result ID Test Result Name Result LOINC Value
39469 PMLR Result No LOINC Needed
MP012 Specimen Type 31208-2
19449 Interpretation 69047-9

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.