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HelpTest Code LAB1234720 Antinuclear Antibodies (ANA), IgG by ELISA with Reflex to ANA HEp-2 Substrate, IgG by IFA and ENA Confirmation
Performing Laboratory
ARUP Laboratories
Specimen Required
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1.0 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)
Specimen Stability Information
Transport: Refrigerated
Stability: After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month (avoid repeated freeze/thaw cycles)
Reject Due To
Non-serum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic, specimens or inclusion of fibrin clots.
Day(s) Performed
Performed: Sun-Sat
Reported: 1-5 days
Method Name
Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody/Semi-Quantitative Multiplex Bead Assay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
CPT Code Information
86038; if reflexed, add 86039; if reflexed, add 86235 x7 and 86225; if reflexed, add 86256
Interpretation
Antinuclear Antibodies (ANA), IgG by ELISA: ANA specimens are screened using enzyme-linked immunosorbent assay (ELISA) methodology. All ELISA results reported as detected are further tested by indirect fluorescent assay (IFA) using HEp-2 substrate with an IgG-specific conjugate. The ANA ELISA screen is designed to detect antibodies against dsDNA, histone, SS-A (Ro), SS-B (La), Smith, Smith/RNP, Scl-70, Jo-1, centromeric proteins, and other antigens extracted from the HEp-2 cell nucleus. ANA ELISA assays have been reported to have lower sensitivities than ANA IFA for systemic autoimmune rheumatic diseases (SARD).
Negative results do not necessarily rule out SARD.
LOINC Code Information
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0050080 | Anti-Nuclear Ab (ANA), IgG by ELISA | 29950-3 |
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds