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HelpTest Code LAB1232674 Brucella Antibody Screen, IgM and IgG, ELISA, Serum
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a sterile, plastic vial.
Useful For
Evaluating patients with suspected brucellosis
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BRCM | Brucella Ab Screen, IgM ELISA, S | No | Yes |
| BRCG | Brucella Ab Screen, IgG ELISA, S | No | Yes |
| BRCI | Brucella Ab Screen Interpretation | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BRUTA | Brucella Ab, Agglutination, S | Yes | No |
Testing Algorithm
If the Brucella antibody screen, IgM or IgG, result is either positive or equivocal, then confirmation by Brucella total antibody agglutination testing will be performed at an additional charge.
Reporting Name
Brucella Ab Screen, IgM/IgG ELISA, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat-inactivated specimen | Reject |
Reference Values
IgG SCREEN
Negative
Reference values apply to all ages.
IgM SCREEN
Negative
Reference values apply to all ages.
Supportive Data
According to the manufacturer's package insert, 127 patient samples testing positive with the Rose-Bengal test were also examined with the Eurroimmun anti-Brucella abortus enzyme-linked immunosorbent assay, and 160 blood donors were tested. Data from these studies were as follows for anti-B abortus:
-IgG: sensitivity, 78.0%; specificity, 98.0%
-IgM: sensitivity, 56.0%; specificity, 98.0%
Day(s) Performed
Tuesday, Thursday
Report Available
Same day/1 to 5Specimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86622 x 2-Brucella antibody, IgG and IgM
86622-Brucella total antibody, agglutination (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BRCMG | Brucella Ab Screen, IgM/IgG ELISA, S | 91140-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BRCM | Brucella Ab Screen, IgM ELISA, S | 24388-1 |
| BRCG | Brucella Ab Screen, IgG ELISA, S | 24387-3 |
| BRCI | Brucella Ab Screen Interpretation | 66485-4 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.