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HelpTest Code LAB1232600 Liver/Kidney Microsome Type 1 Antibodies, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Liver/Kidney Microsome Type 1 Ab, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat treated | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Specimen Minimum Volume
Serum: 0.4 mL
Special Instructions
Day(s) Performed
Monday, Wednesday, Friday
Specimen Retention Time
14 daysReport Available
2 to 4 daysReference Values
Negative: ≤20.0 Units
Equivocal: 20.1-24.9 Units
Positive: ≥25.0 Units
Reference values apply to all ages.
Useful For
Evaluation of patients with liver disease of unknown etiology
Evaluation of patients with suspected autoimmune hepatitis
Testing Algorithm
For information see First-Line Screening for Autoimmune Liver Disease Algorithm.
CPT Code Information
86376
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LKM | Liver/Kidney Microsome Type 1 Ab, S | 32220-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LKM | Liver/Kidney Microsome Type 1 Ab, S | 32220-6 |
Method Description
Purified full-length recombinant human cytochrome P450 2D6 antigen is bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Pre-diluted controls and diluted patient sera are added to separate wells, allowing any liver/kidney microsomal antibodies type 1 present to bind to the immobilized antigen. Unbound sample is washed away, and an enzyme labeled anti-human IgG antibody (conjugate) is added to each well. A second incubation allows the enzyme labeled anti-human IgG antibody to bind any patient antibodies, which have become attached to the microwells. After washing away any unbound enzyme labeled anti-human IgG antibody, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. The assay is evaluated by spectrophotometrically measuring and comparing the color intensity that develops in the patient wells with the color in the calibrator wells.(Package insert: INOVA Diagnostics, Inc.; Revision 13; 10/2018)
Forms
If not ordering electronically, complete, print, and send 1 of the following with the specimen:
-Gastroenterology and Hepatology Test Request (T728)
-General Request (T239)