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HelpTest Code LAB1231916 Paroxysmal Nocturnal Hemoglobinuria, PI-Linked Antigen, Blood
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
PNH, PI-Linked AG, BSpecimen Type
Whole bloodShipping Instructions
Specimen must arrive within 72 hours of collection.
Specimen Required
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA)
Specimen Volume: 2.6 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Reject Due To
| Gross hemolysis | Reject |
| Fully clotted | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Ambient (preferred) | 72 hours |
| Refrigerated | 72 hours |
Specimen Minimum Volume
1 mL
Day(s) Performed
Monday through Saturday
Specimen Retention Time
14 daysReport Available
1 to 3 daysReference Values
An interpretive report will be provided.
RED BLOOD CELLS:
PNH RBC-Partial Antigen loss: 0.00-0.99%
PNH RBC-Complete Antigen loss: 0.00-0.01%
PNH Granulocytes: 0.00-0.01%
PNH Monocytes: 0.00-0.05%
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FCIMS | Flow Cytometry Interp, 9-15 Markers | No, (Bill Only) | Yes |
Useful For
Screening for and confirming the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH)
Monitoring patients with PNH
CPT Code Information
88184-Flow cytometry, RBC x 1
88184-Flow cytometry, WBC x 1
88185-Flow cytometry, additional marker (each), RBC x 1
88185-Flow cytometry, additional marker (each), WBC x 6
88188-Flow Cytometry Interpretation, 9-15 Markers x 1
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PLINK | PNH, PI-Linked AG, B | 90735-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CK079 | Interpretation | 90739-4 |
| CK080 | PNH RBC-Partial Ag Loss | In Process |
| CK081 | PNH RBC-Complete Ag Loss | 90738-6 |
| CK082 | PNH Granulocytes | 90737-8 |
| CK083 | PNH Monocytes | 90736-0 |
Method Description
Flow cytometric immunophenotyping of peripheral blood (white blood cells [WBCs] and red blood cells [RBCs]) is performed using the following antibodies:
RBC: CD235a, CD59
WBC: CD14, CD15, CD16, CD24, CD33, CD45, and FLAER
This assay evaluates the presence or absence of glycosylphosphatidylinositol (GPI)-linked proteins using monoclonal antibodies directed against CD235, CD33, and CD15 to isolate different cell lineages. GPI-linked proteins that are checked within different lineages include CD14 for monocytes, CDs 16 and 24 for granulocytes, and CD59 for RBC. Fluorescent aerolysin, a fluorescently labeled inactive variant of the protein aerolysin, binds selectively to GPI anchors and is evaluated for presence or absence of expression on WBCs. In addition, this test will detect a partial loss of CD59 on RBCs (type II RBC).
Individuals without paroxysmal nocturnal hemoglobinuria have normal expression of all GPI-linked antigens on peripheral blood and leukocytes and erythrocytes.(Devalet B, Mullier F, Chatelain B, Dogne JM, Chatelain C. Pathophysiology, diagnosis, and treatment of paroxysmal nocturnal hemoglobinuria: a review. Eur J Haematol. 2015;95(3):190-198. doi:10.1111/ejh.12543)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: