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HelpTest Code LAB1231886 RNA Polymerase III Antibodies, IgG, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
RNA Polymerase III Ab, IgG, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.3 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimen | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Specimen Minimum Volume
See Specimen Required
Day(s) Performed
Tuesday, Thursday
Specimen Retention Time
14 daysReport Available
2 to 7 daysReference Values
<20.0 U (negative)
20.0-39.9 U (weak positive)
40.0-80.0 U (moderate positive)
>80.0 U (strong positive)
Useful For
Evaluating patients suspected of having systemic sclerosis as part of systemic sclerosis criteria antibody tests
Providing diagnostic and prognostic information in patients with systemic sclerosis
CPT Code Information
83516
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| RNAP | RNA Polymerase III Ab, IgG, S | 79182-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| RNAP | RNA Polymerase III Ab, IgG, S | 79182-2 |
Method Description
The immunodominant fragment of RNA polymerase III antigen is derived from recombinant DNA technology. Purified RNA polymerase III antigen is adsorbed to the wells of a polystyrene microtiter plate under conditions that preserve the antigen in its antigenic state. Prediluted controls and diluted patient sera are added to separate wells. Unbound sample is washed away, and an enzyme-labeled antihuman IgG conjugate is added to each well. After incubation and washing away of unbound enzyme-labeled antihuman IgG, the bound conjugate is measured by adding a chromogenic substrate. The intensity of the absorbance produced is measured with an automated microwell plate reader. Results are calculated by comparison to a single-point calibrator.(Package insert: QUANTA Lite RNA Pol III. INOVA Diagnostics; 05/2019)