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Test Code LAB122100 Nicotine and Metabolites, Random, Urine

Important Note

Mayo's test code: NICOU

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Nicotine and Metabolites, U

Specimen Type

Urine


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 5-mL tube

Specimen Volume: 3 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.


Reject Due To

Gross hemolysis OK
Gross icterus OK

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 28 days
  Frozen  365 days
  Ambient  28 days

Specimen Minimum Volume

2 mL

Day(s) Performed

Monday through Sunday

Specimen Retention Time

2 weeks

Report Available

2 to 5 days

Reference Values

Non-tobacco user with no passive exposure:

Nicotine <5.0 ng/mL

 

Cotinine

<5.0 ng/mL

 

Anabasine

<2.0 ng/mL

 

Nornicotine

<2.0 ng/mL

Useful For

Monitoring tobacco use

 

Monitoring patients on nicotine-replacement therapy for concurrent use of tobacco products

CPT Code Information

G0480

80323 (if appropriate for select payers)

Clinical Toxicology CPT Code Client Guidance

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NICOU Nicotine and Metabolites, U 43125-4

 

Result ID Test Result Name Result LOINC Value
82510 Nicotine 3854-7
21321 Cotinine 10366-3
21324 Nornicotine 33917-6
21323 Anabasine 33915-0

Method Description

Nicotine and metabolites are extracted from urine by solid-phase extraction techniques. The extract eluate is quantified by high-performance liquid chromatography tandem mass spectrometry.(Moyer TP, Charlson JR, Enger RJ, et al. Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem. 2002;48[9]:1460-1471; Oh J, Park MS, Chun MR, et al. A simple and high-throughput LC-MS/MS method for simultaneous measurement of nicotine, cotinine, 3-OH cotinine, nornicotine, and anabasine in urine and its application in the general Korean population. J Anal Toxicol. 2022:46[1]:25-36. doi:10.1093/jat/bkaa177)

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.