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HelpTest Code LAB1176 Mitochondrial Antibodies (M2), Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Mitochondrial Ab, M2, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimens | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Specimen Minimum Volume
Serum: 0.4 mL
Day(s) Performed
Monday through Saturday
Specimen Retention Time
14 daysReport Available
2 to 3 daysReference Values
Negative: <0.1 Units
Borderline: 0.1-0.3 Units
Weakly positive: 0.4-0.9 Units
Positive: ≥1.0 Units
Reference values apply to all ages.
Useful For
Establishing the diagnosis of primary biliary cholangitis
This test is not useful for indicating the stage or prognosis of the disease or for monitoring the course of the disease.
CPT Code Information
86381
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AMA | Mitochondrial Ab, M2, S | 51715-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AMA | Mitochondrial Ab, M2, S | 51715-1 |
Method Description
A recombinant pyruvate dehydrogenase complex-E2 (M2) antigen for detection of antibodies against M2 is attached to the surface of a microplate. Diluted patient serum, standards, or controls are added to the wells, and the M2 specific IgG and IgM antibodies, if present, bind to the antigen. All unbound human antibodies are washed away, and a conjugate of enzyme-labeled polyclonal antibody to human IgG and IgM is added. The enzyme conjugate binds to the antibody complex. Excess enzyme-conjugate is washed away, and substrate is added. After a specified time, the enzyme reaction is stopped. The intensity of the color generated is proportional to the amount of anti-M2 IgG and/or IgM antibody in the sample. The results are read by a spectrophotometer producing a direct measurement of the anti-M2 IgG and IgM antibodies in the serum. Testing is performed on the Agility instrument by Dynex.(Package insert: Kallestad Anti-Mitochondrial Kit. Bio-Rad Laboratories, Inc; 10/2014)
Supportive Data
Testing performed in the Antibody Immunology Laboratory of the antimitochondrial antibody-M2 by enzyme immunoassay (EIA) revealed a false-positive rate of less than 2% in 196 normal samples, and overall concordance compared with indirect immunofluorescence (IFA) of 90% on sera from the Mayo primary biliary cholangitis (PBC) Serum Bank. Ten discordant results were obtained (negative by EIA and positive by IFA). Seven of the 10 patients had no histologic evidence of PBC on liver biopsy.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)