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Test Code HSTNI Troponin I, High Sensitivity, Plasma


Specimen Required


Collection Container/Tube:

Preferred: Mint green top (lithium heparin gel)

Acceptable: Green top (lithium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Plasma gel tube should be centrifuged within 2 hours of collection.

2. Green-top tube should be centrifuged, and the plasma aliquoted into a plastic vial within 2 hours of collection.


Forms

If not ordering electronically, complete, print, and send a Cardiovascular Test Request Form (T724) with the specimen.

Useful For

Excluding the diagnosis of acute coronary syndromes

 

Explaining troponin T elevations related to skeletal myopathy and/or assay interferences

Reporting Name

Troponin I, High Sensitivity, P

Specimen Type

Plasma

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Frozen (preferred) 180 days
  Refrigerated  48 hours
  Ambient  4 hours

Reject Due To

Gross hemolysis Reject

Reference Values

Males ≥18 years: ≤20 ng/L

Females ≥18 years: ≤15 ng/L

 

Reference values have not been established for patients younger than 18 years old.

Method Description

The Access hsTnI (high sensitivity troponin I) assay is a two-site immunoenzymatic (sandwich) assay. Monoclonal anti-cTnI (cardiac troponin I) antibody conjugated to alkaline phosphatase is added to a reaction vessel along with a surfactant-containing buffer and sample. After a short incubation, paramagnetic particles coated with monoclonal anti-cTnI antibody are added. The human cTnI binds to the anti-cTnI antibody on the solid phase, while the anti-cTnI antibody-alkaline phosphatase conjugate reacts with different antigenic sites on the cTnI molecules. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of cTnI in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve.(Package insert: ACCESS hsTnI. Beckman Coulter; 10/2021)

Day(s) Performed

Monday, Wednesday, Friday

Report Available

2 to 3 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84484

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HSTNI Troponin I, High Sensitivity, P 89579-7

 

Result ID Test Result Name Result LOINC Value
HSTNI Troponin I, High Sensitivity, P 89579-7