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Test Code HEVG Hepatitis E Virus IgG Antibody, Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

HEV IgG Ab, S

Specimen Type

Serum SST


Necessary Information


Date of collection is required.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into a plastic vial.


Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Specimen Stability Information

Specimen Type Temperature Time
Serum SST Frozen (preferred)
  Refrigerated  24 hours

Specimen Minimum Volume

See Specimen Required

Day(s) Performed

Wednesday, Friday

Specimen Retention Time

14 days

Report Available

1 to 7 days

Reference Values

Negative

Useful For

Diagnosis of past exposure to hepatitis E virus

Testing Algorithm

For information see Hepatitis E: Diagnostic Testing Algorithm.

CPT Code Information

86790

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HEVG HEV IgG Ab, S 49693-5

 

Result ID Test Result Name Result LOINC Value
86211 HEV IgG Ab, S 49693-5

Method Description

This is a qualitative, in vitro test for the detection and identification of IgG antibodies specifically against to hepatitis E virus (HEV) in human serum. This assay is a screening test based on the principle of an indirect sandwich enzyme-linked immunosorbent assay.

 

Highly purified recombinant HEV-ORF2 viral antigens (specific for HEV genotypes 1 and 3) are fixed to microplate wells. Diluted patient serum samples are incubated in the wells, in which antibodies bind specifically to the HEV recombinant antigens coating the surface of the wells. Unbound antibodies are then washed away. Anti-human immunoglobulin antibodies (IgG), which are coupled to horseradish peroxidase, are then added to the wells and incubated. Unbound conjugate antibodies are then washed away. Specifically, bound antibodies are detected by a peroxidase-catalyzed color reaction. Intensity of the color, as measured with a photometric analyzer, is proportionate to the quantity of bound HEV IgG antibodies present in the serum specimen.(Unpublished Mayo method)

Forms

If not ordering electronically, complete, print, and send 1 of the following:

-Gastroenterology and Hepatology Test Request (T728)

-Infectious Disease Serology Test Request (T916)

-Microbiology Test Request (T244)