Method Description

The Lumipulse G GFAP Immunoreaction is an assay system for the quantitative measurement of glial fibrillary acidic protein (GFAP) in plasma specimens based on chemiluminescent enzyme immunoassay technology by a specific two-step immunoassay method on the Lumipulse G System. The specimen and assay-specific diluent are added to the antibody-coated particle solution. The GFAP in the specimen specifically binds to anti-GFAP monoclonal mouse antibody on the particles and antigen-antibody immunocomplexes are formed. The particles are washed and rinsed to remove unbound materials. Alkaline phosphatase-labeled anti-GFAP monoclonal antibodies specifically bind to immunocomplexes on the particles. The particles are washed and rinsed to remove unbound materials. The substrate solution is added and mixed with the particles. 3-(2'-Spiroadamantyl)-4-methoxy-4-(3"-phosphoryloxy)-phenyl-1,2-dioxetane (AMPPD) contained in the substrate solution is dephosphorylated by the catalysis of alkaline phosphatase indirectly conjugated to particles. Luminescence (at a maximum wavelength of 477 nm) is generated by the cleavage reaction of dephosphorylated AMPPD. The luminescent signal reflects the amount of GFAP present in the sample.(Package insert: Lumipulse G GFAP. Fujirebio Inc; ver 1, 07/2024)