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Test Code GENPA Gentamicin, Peak, Serum

Useful For

Monitoring adequacy of drug clearance during gentamicin therapy

Reporting Name

Gentamicin, Peak, S

Specimen Type

Serum


Specimen Required


Collection Container/Tube: 

Preferred: Serum gel 

Acceptable: Red top 

Submission Container/Tube: Plastic vial 

Specimen Volume: 0.5 mL 

Collection Instructions:  

1. Serum gel tubes should be centrifuged within 2 hours of collection. 

2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  14 days
  Ambient  72 hours

Reject Due To

Gross hemolysis Reject

Reference Values

Peak: 3.0-12.0 mcg/mL

Toxic peak: >12.0 mcg/mL

Method Description

The CEDIA Gentamicin II Assay uses recombinant DNA technology to produce a unique homogeneous enzyme immunoassay system. The assay is based on the bacterial enzyme beta-galactosidase, which has been genetically engineered into two inactive fragments: enzyme acceptor and enzyme donor . These fragments spontaneously reassociate to form fully active enzyme that, in the assay format, cleaves a substrate, generating a color change that can be measured spectrophotometrically. In the assay, analyte in the sample competes with analyte conjugated to one inactive fragment of beta-galactosidase for antibody binding site. If analyte is present in the sample, it binds to antibody, leaving the inactive enzyme fragments to form active enzymes. If analyte is not present in the sample, antibody binds to analyte conjugated on the inactive fragment, inhibiting the reassociation of inactive beta-galactosidase fragments, and no active enzyme is formed. The amount of active enzyme formed, and resultant absorbance change are directly proportional to the amount of drug present in the sample.(Package insert: CEDIA Gentamicin II Assay. Thermo Scientific; 09/2022)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 day

Specimen Retention Time

1 week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80170

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GENPA Gentamicin, Peak, S 3663-2

 

Result ID Test Result Name Result LOINC Value
GENPA Gentamicin, Peak, S 3663-2

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.