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Test Code FENTS Fentanyl, Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Fentanyl and Metabolites, S

Specimen Type

Serum Red


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.3 mL

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Frozen (preferred) 28 days
  Refrigerated  14 days
  Ambient  72 hours

Specimen Minimum Volume

1.25 mL

Day(s) Performed

Tuesday, Thursday

Specimen Retention Time

14 days

Report Available

2 to 7 days

Reference Values

Fentanyl:

Average serum fentanyl concentrations 24 hours after application of transdermal patch:

25 mcg/hour: 0.3-1.2 ng/mL

50 mcg/hour: 0.6-1.8 ng/mL

75 mcg/hour: 1.1-2.6 ng/mL

100 mcg/hour: 1.9-3.8 ng/mL

(Baselt RC. Disposition of Toxic Drugs and Chemical in Man. 12th ed. Biomedical Publications; 2020)

 

Norfentanyl:

Reference range: Not established

 

Cutoff concentrations by liquid chromatography tandem mass spectrometry:

Fentanyl: 0.05 ng/mL

Norfentanyl: 0.25 ng/mL

Useful For

Monitoring fentanyl therapy

CPT Code Information

G0480

80354 (if appropriate for select payers)

Clinical Toxicology CPT Code Client Guidance

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FENTS Fentanyl and Metabolites, S 81275-0

 

Result ID Test Result Name Result LOINC Value
31829 Norfentanyl 11074-2
31830 Fentanyl 3636-8
31832 Chain of Custody 77202-0

Method Description

Fentanyl is isolated from serum using a liquid/liquid extraction. The solvent is dried, and the analytes are reconstituted with mobile phase. Analysis is performed by liquid chromatography tandem mass spectrometry using selected ion monitoring.(Unpublished Mayo method)

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.