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HelpTest Code CYCL Cyclospora Stain, Feces
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Cyclospora StainSpecimen Type
FecalOrdering Guidance
This test is intended specifically for the detection of Cyclospora.
If Cryptosporidium is suspected, the specific enzyme-linked immunosorbent assay for this organism is recommended; order CRYPS / Cryptosporidium Antigen, Feces.
GIP / Gastrointestinal Pathogen Panel, PCR, Feces should be considered for the detection of Cyclospora, Cryptosporidium, and other common infectious causes of diarrhea.
Specimen Required
Patient Preparation: Patient should avoid use of antidiarrheal medication (eg, loperamide [Imodium-AD] or Pepto-Bismol).
The presence of barium will interfere with this test.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Preserved stool
Supplies:
-ECOFIX Stool Transport Vial (Kit) (T219)
-Formalin-Meridian 10% Buffered Neutral (T466)
Container/Tube:
Preferred: ECOFIX Stool Transport Vial (Kit)
Acceptable: 10% Buffered Formalin Stool Transport (Kit), Sodium Acetate Formalin (SAF)
Specimen Volume: 10 g
Specimen Stability Information: Ambient 21 days (preferred)/Refrigerated 21 days
Acceptable
Specimen Type: Unpreserved stool
Supplies: Stool container, Small (Random), 4 oz (T288)
Container/Tube: Stool container
Specimen Volume: 5 g
Specimen Stability Information: Refrigerated 3 days (preferred)
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Fecal | Varies | |
Specimen Minimum Volume
Preserved stool: 1 g
Unpreserved stool: 2 g
Special Instructions
Day(s) Performed
Monday through Saturday
Specimen Retention Time
3 daysReport Available
2 to 4 daysReference Values
Negative
If positive, reported as Cyclospora cayetanensis detected.
Useful For
Identifying Cyclospora cayetanensis as a cause of infectious gastroenteritis
Testing Algorithm
For other diagnostic tests that may be of value in evaluating patients with diarrhea; the following are available:
CPT Code Information
87015
87207
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CYCL | Cyclospora Stain | 50313-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CYCL | Cyclospora Stain | In Process |
Method Description
A thin smear of the fecal concentrate is placed on a slide and allowed to air dry. The slide is then placed in acidic alcohol for 5 minutes, followed by washing with cold tap water. The slide is then placed in a Coplin jar containing safranin solution in acidified water and heated in a microwave at full power for 1 minute. Excess stain is washed off with tap water, and the slide is placed in a Coplin jar containing the counterstain (aqueous solution of either 1% methylene blue or 1% malachite green) for 1 minute. After a final rinse in tap water, the slide is dried, and a coverslip is applied. The slide is examined microscopically using high-power with oil immersion. The oocysts of Cyclospora will be clear to pink and have a somewhat refractile quality.(Visvesvara GS, Moura H, Kovacs-Nace E, Wallace S, Eberhard ML: Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J Clin Microbiol 1997;35:730-733; Mathison BA, Pritt BS: Cyclosporiasis-Updates on clinical presentation, pathology, clinical diagnosis, and treatment. Microorganisms. 2021 Sep 2;9(9):1863. doi: 10.3390/microorganisms9091863)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Microbiology Test Request (T244)