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HelpTest Code BFLA1 Lipid Analysis, Body Fluid
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Sources:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, JP drain)
-Pericardial
Acceptable Source:
Collection Container/Tube: Sterile container, no additive
Submission Container/Tube: Plastic vial
Specimen Volume: 3 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Useful For
Distinguishing between chylous and nonchylous effusions
Reporting Name
Lipid Analysis, BFSpecimen Type
Body FluidSpecimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Body Fluid | Frozen (preferred) | 30 days |
| Refrigerated | 7 days | |
| Ambient | 24 hours |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
| Breast milk Nasal secretions Gastric secretions Bronchoalveolar lavage (BAL or bronchial washings) Colostomy/ostomy Amniotic fluid Feces Saliva Sputum Urine Spinal fluid Synovial fluid Vitreous fluid |
Reject |
Reference Values
An interpretive report will be provided.
Method Description
Electrophoresis:
Samples are analyzed for qualitative interpretation of chylomicrons and plasma lipoproteins using a lipid specific Sudan black stain.(Package insert: Hydragel 7, 15 and 30 LIPO + Lp(a). Sebia Inc; 07/2020)
Cholesterol:
Cholesterol esters are cleaved by the action of cholesterol esterase to yield free cholesterol and fatty acids. Cholesterol oxidase then catalyzes the oxidation of cholesterol to cholest-4-en-3-one and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide formed effects the oxidative coupling of phenol and 4-aminophenazone to form a red quinone-imine dye. The color intensity of the dye formed is directly proportional to the cholesterol concentration. It is determined by measuring the increase in absorbance.(Package insert: Cholesterol Gen2 Reagent. Roche Diagnostics; V 16.0, 10/2023)
Triglycerides:
Samples analyzed for triglycerides are measured by an automated enzymatic method. The chemistry includes hydrolysis of the triglycerides and phosphorylation of the resulting glycerol.(Package insert: Triglycerides Reagent. Roche Diagnostics; V13.0, 03/2022)
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysSpecimen Retention Time
3 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82664-Electrophoretic technique, not elsewhere specified (Chylomicrons and lipoproteins)
84311-Spectrophotometry, analyte not specified (Cholesterol)
84478-Triglycerides
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BFLA1 | Lipid Analysis, BF | 94872-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| FLD28 | Fluid Type: | 14725-6 |
| BFCHO | Cholesterol, BF | 12183-0 |
| BFTRG | Triglycerides, BF | 12228-3 |
| BFCMT | Comment | 21025-2 |