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HelpTest Code BA190 BCR::ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
BCR/ABL1, p190, Quant, MonitorSpecimen Type
VariesOrdering Guidance
This test is intended for therapeutic monitoring of patients with known e1a2 BCR::ABL1 (p190) fusion form and should not be used to screen for BCR::ABL1 fusion forms at the time of diagnosis.
To screen for BCR::ABL1 fusion forms at diagnosis, order one of the following:
BADX / BCR::ABL1, Qualitative, Diagnostic Assay, Varies
BCRFX / BCR::ABL1 Qualitative Diagnostic Assay with Reflex to BCR::ABL1 p190 Quantitative Assay or BCR::ABL1 p210 Quantitative Assay, Varies.
To monitor patients carrying BCR::ABL1 fusion forms coding for p210 (e13/e14a2), order BCRAB / BCR::ABL1, p210, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Chronic Myeloid Leukemia (CML), Varies.
To monitor patients carrying rare BCR::ABL1 fusion forms coding for e19a2, e13/e14a3, e1a3, e6a2, e19a3, e8a2, e12a2, e6a3, e8a3, and e12a3, order BARQ / BCR::ABL1, Rare Fusion Monitoring, Quantitative, Varies.
Shipping Instructions
Refrigerate specimens must arrive within 5 days of collection, and ambient specimens must arrive within 72 hours of collection. Collect and package specimen as close to shipping time as possible.
Necessary Information
Pertinent clinical history including patient diagnosis of chronic myeloid leukemia or other BCR::ABL1-positive neoplasm information is required.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Whole blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 10 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as whole blood.
Specimen Type: Bone marrow
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Reject Due To
| Gross hemolysis | Reject |
| Moderately to severely clotted | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 5 days | PURPLE OR PINK TOP/EDTA |
| Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Specimen Minimum Volume
Whole blood: 8 mL; Bone marrow: 2 mL
Special Instructions
Day(s) Performed
Monday through Friday
Specimen Retention Time
Whole blood/Bone marrow: 2 weeks; Extracted RNA 3 monthsReport Available
4 to 8 daysReference Values
The presence or absence of the BCR::ABL1 messenger RNA fusion form producing the p190 fusion protein is reported. If positive, the level is reported as the ratio of BCR::ABL1 (p190) transcript to ABL1 transcript in the form of a percentage.
Useful For
Therapeutic monitoring of BCR::ABL1 p190 (e1a2)-positive hematologic neoplasms (eg, acute lymphoblastic leukemia, chronic myeloid leukemia)
This test should not be used to screen for BCR::ABL1 fusion forms at the time of diagnosis or quantitative therapeutic monitoring of p210 or rare fusion forms.
Testing Algorithm
For information see BCR::ABL1 Ordering Guide for Blood and Bone Marrow.
CPT Code Information
81207
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BA190 | BCR/ABL1, p190, Quant, Monitor | 21823-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 39470 | BCR/ABL1 p190 Result | No LOINC Needed |
| MP002 | Specimen Type | 31208-2 |
| 19765 | Interpretation | 69047-9 |
Method Description
Total RNA is extracted and reverse transcribed to complementary DNA. Quantitative real time polymerase chain reaction is performed and p190/ABL quantitative levels are determined using TaqMan-type probe technology. The data is analyzed for relative quantification with calibrator normalization and efficiency correction. The reference gene, ABL1, is used to control for RNA degradation in the sample and the calibrator is used to control for inter-run variations. A normalized ratio of BCR::ABL1 (p190) mRNA:ABL1 mRNA is obtained and reported in the form of a percentage.(Unpublished Mayo method)
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.