Home
HelpTest Code ANIDE Organism Referred for Identification, Anaerobic Bacteria
Infectious
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Organism Ref for ID, Anaerobic BactSpecimen Type
VariesOrdering Guidance
If susceptibility testing is needed; order MMLSA / Antimicrobial Susceptibility, Anaerobic Bacteria, Minimal Inhibitory Concentration, Varies also. If susceptibilities are not appropriate, MMLSA will be canceled at report time.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance, if appropriate.
Necessary Information
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
Specimen Required
Specimen Type: Pure culture of organism from a source not normally colonized by anaerobes
Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds
Supplies:
-Anaerobic Transport Tube (T588)
-Infectious Container, Large (T146)
Container/Tube:
Preferred: Anaerobic transport tube
Acceptable: Thioglycollate broth or any other suitable anaerobic transport system
Collection Instructions:
1. Perform isolation of infecting bacteria.
2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
Reject Due To
| Agar plate | Reject |
| Received frozen | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Specimen Minimum Volume
See Specimen Required
Special Instructions
Day(s) Performed
Monday through Sunday
Specimen Retention Time
30 daysReport Available
8 to 14 daysReference Values
Identification of organism
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALA | Id MALDI-TOF Mass Spec Anaerobe | No, (Bill Only) | No |
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
| COMM | Identification Commercial Kit | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| GID | Bacteria Identification | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| SALS | Serologic Agglut Method 1 Ident | No, (Bill Only) | No |
| EC | Serologic Agglut Method 2 Ident | No, (Bill Only) | No |
| SHIG | Serologic Agglut Method 3 Ident | No, (Bill Only) | No |
| STAP | Identification Staphylococcus | No, (Bill Only) | No |
| STRP | Identification Streptococcus | No, (Bill Only) | No |
| SIDC | Ident Serologic Agglut Method 4 | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
Useful For
Identifying anaerobic bacteria involved in human infections
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will be identified and billed as appropriate.
CPT Code Information
87076-Organism ref for ID, anaerobic bact
87076-Id MALDI-TOF mass spec anaerobe (if appropriate)
87076-Anaerobe Ident (if appropriate)
87153-Anaerobe ident by sequencing (if appropriate)
87077-Identification commercial kit (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria identification (if appropriate)
87077-Additional identification procedure (if appropriate)
87077-Identification Staphylococcus (if appropriate)
87077-Identification Streptococcus (if appropriate)
87147 x 3-Serologic agglut method 1 ident (if appropriate)
87147-Serologic agglut method 2 ident (if appropriate)
87147 x 4-Serologic agglut method 3 ident (if appropriate)
87147 x 2-6-Serologic Agglut Method 4 Ident (if appropriate)
87153-Aerobe ident by sequencing (if appropriate)
87798-Identification by PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ANIDE | Organism Ref for ID, Anaerobic Bact | 20878-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ANIDE | Organism Ref for ID, Anaerobic Bact | 20878-5 |
Method Description
Appropriately transported organisms are inoculated onto blood agar plates and into thioglycollate broth tubes. After 48 hours of incubation at 35° C in an anaerobic atmosphere, colonies are identified using one or a combination of the following techniques: Gram stain, use of various differential media, aero tolerance testing, conventional biochemical tests, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, or 16S ribosomal RNA gene sequencing.(Procop, GW, Church DL, Hall GS, et al. The anaerobic bacteria. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Lippincott, Williams and Wilkins; 2017:chap 16)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.