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Test Code ANAP Anaplasma phagocytophilum (Human Granulocytic Ehrlichiosis) Antibody, Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Reporting Name

Anaplasma phagocytophilum Ab, IgG,S

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-inactivated specimen Reject

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Specimen Minimum Volume

0.4 mL

Day(s) Performed

Monday through Friday

Specimen Retention Time

14 days

Report Available

1 to 3 days

Reference Values

<1:64

Reference values apply to all ages.

Useful For

As an adjunct in the diagnosis of human granulocytic ehrlichiosis (anaplasmosis)

 

Seroepidemiological surveys of the prevalence of the infection in certain populations

Testing Algorithm

For information see Acute Tick-borne Disease Testing Algorithm.

CPT Code Information

86666

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANAP Anaplasma phagocytophilum Ab, IgG,S 23877-4

 

Result ID Test Result Name Result LOINC Value
81157 Anaplasma phagocytophilum Ab, IgG,S 23877-4

Method Description

The patient's serum is diluted and is placed in microscopic slide wells that have been coated with Anaplasma phagocytophilum-infected cells. After incubation, the slides are washed and a fluorescein-isothiocyanate conjugate is added to each well. The slides are then read using a fluorescence microscope and significant fluorescent staining of intracellular organisms constitutes a positive reaction.(Dumler JS, Asanovich KM, Bakken JS, Richter P, Kimsey R, Madigan JE. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic Ehrlichia. J Clin Microbiol. 1995;33[5]:1098-1103; Pancholi P, Kolbert CP, Mitchell PD, et al. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis. 1995;172[4]:1007-1012; package insert: Anaplasma phagocytophilum IFA IgG. DiaSorin Molecular; Rev. I, 08/12/2016)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.