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HelpTest Code ANAES Bacterial Culture, Anaerobic with Antimicrobial Susceptibilities, Varies
Shipping Instructions
Specimen should arrive within 72 hours of collection.
Necessary Information
Specimen source is required.
Specimen Required
Supplies: Anaerobic Transport Tube (T588)
Acceptable Sources: Deep tissues, sterile body fluids, abscesses, percutaneous transtracheal aspirates, suprapubic aspirations, or wounds
Collection Instructions: Specimen should be obtained by using a needle and syringe from a source not normally colonized by anaerobes.
Useful For
Diagnosing anaerobic bacterial infections
Directing antimicrobial therapy for anaerobic infections
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| RMALA | Id MALDI-TOF Mass Spec Anaerobe | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
| PCRID | Identification by PCR | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| BLA | Beta Lactamase | No, (Bill Only) | No |
| BATTA | Anaerobe Suscep Battery | No, (Bill Only) | No |
| SANA | Anaerobe Suscep per agent | No, (Bill Only) | No |
| MECAB | mecA PCR Test, Bill Only | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge. All bacterial organisms submitted will automatically have susceptibility testing performed and billed as appropriate. Antimicrobial agents appropriate to the organism and specimen source will be tested according to Mayo Clinic's practice and the laboratory's standard operating procedures.
See Anaerobic Bacteria Antimicrobials for a listing of the antimicrobials routinely tested in our laboratory as well as antimicrobials that may be tested upon request. Call 800-533-1710 and ask to speak to the Bacteriology Anaerobe Laboratory if the organism or antimicrobial of interest is not listed in this table.
For information see Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology.
Special Instructions
Reporting Name
Bacterial Culture, Anaerobic + SuscSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | 72 hours |
Reject Due To
| Swab | Reject |
Reference Values
No growth
Identification of probable pathogens
Susceptibility results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as clinical breakpoints) are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to breakpoint setting organizations, either the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), as applicable.
In some instances, an interpretive category cannot be provided based on available data; therefore, the following comment will be included on the report: There are no established interpretive guidelines for agents reported without interpretations.
For information regarding CLSI and EUCAST susceptibility interpretations, see Susceptibility Interpretative Category Definitions.
Method Description
Appropriate specimens are inoculated onto blood agar, phenylethyl alcohol agar, and lysed blood agar containing gentamicin and vancomycin and into thioglycollate broth tubes. After 48 hours of incubation at 35° C in an anaerobic atmosphere, colonies are identified using 1 or a combination of the following techniques: Gram stain, use of various differential media, aerotolerance testing, conventional biochemical tests, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, real-time polymerase chain reaction, or 16S ribosomal RNA gene sequencing.(Procop GW, Church DL, Hall GS, et al. The anaerobic bacteria. In: Koneman's Color Atlas and Textbook of Diagnostic Microbiology. 7th ed. Wolters; 2017:983-1073)
An agar dilution method is used for routine susceptibility testing. The antimicrobial is added to agar in various concentrations depending upon levels attainable in serum. A standardized suspension of the organism is applied to the agar plates, which are incubated anaerobically for 42 to 48 hours at 35 to 37° C. The end point is that in which a marked reduction occurs in the appearance of growth on the test plate as compared to that of growth on the control plate. Examples of marked change include a change from confluent growth to a haze, less than 10 tiny colonies, or 1 to 3 normal-sized colonies.(Clinical and Laboratory Standards Institute [CLSI]. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. 9th ed. CLSI standard M11. CLSI; 2018)
Day(s) Performed
Monday through Sunday
Report Available
14 to 20 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87075-Bacterial Culture, Anaerobic
87076-Anaerobe Ident (if appropriate)
87076-Id MALDI-TOF Mass Spec Anaerobe (if appropriate)
87153-Anaerobe Ident by Sequencing (if appropriate)
87150-Identification by PCR (if appropriate)
87176-Tissue Processing (if appropriate)
87185-Beta Lactamase (if appropriate)
87186-Antimicrobial Susceptibility, Anaerobic Bacteria, MIC (if appropriate)
87181-Anaerobe Susceptibility per agent (if appropriate)
87150-mecA PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ANAES | Bacterial Culture, Anaerobic + Susc | 635-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ANAES | Bacterial Culture, Anaerobic + Susc | 635-3 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.