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HelpTest Code AMLPC Amylase, Pancreatic Cyst Fluid
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Amylase, Pancreatic CystSpecimen Type
Pancreatic Cyst FluidOrdering Guidance
For other body fluid specimens (eg, peritoneal, pleural), order AMBF / Amylase, Body Fluid. Testing will be changed to AMBF if this test is ordered on any fluid other than pancreatic fluid.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plain vial
Specimen Volume: 1 mL
Additional Information: A minimum of 0.5 mL is required for testing; specimens less than 0.5 mL may be rejected.
Reject Due To
| Gross hemolysis | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Pancreatic Cyst Fluid | Frozen (preferred) | 30 days |
| Ambient | 7 days | |
| Refrigerated | 7 days |
Specimen Minimum Volume
0.5 mL
Day(s) Performed
Monday through Saturday
Specimen Retention Time
12 monthsReport Available
1 to 3 daysReference Values
An interpretive report will be provided.
Useful For
Aiding in distinguishing between pseudocysts and other types of pancreatic cysts when used in conjunction with imaging studies, cytology, and other pancreatic cyst fluid tumor markers
CPT Code Information
82150
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AMLPC | Amylase, Pancreatic Cyst | 48996-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AMYPC | Amylase, Pancreatic Cyst | 48996-3 |
| SITE6 | Site | 39111-0 |
Method Description
The Roche amylase method is an enzymatic colorimetric test using 4,6-ethylidene (G7)-p-nitrophenol (G1)-alpha, D-maltoheptaoside (ethylidene-G7PNP) as a substrate. Human salivary and pancreatic amylases (alpha-amylase) convert the substrate at approximately the same rate. The alpha-amylase cleaves the substrate into G2, G3, G4 p-nitrophenol (PNP) fragments. The G2, G3, and G4 PNP fragments are further hydrolyzed by an alpha-glucosidase to yield PNP and glucose. The rate of increase in absorbance at 415 nm (measuring the increase in PNP) is directly proportional to amylase activity.(Package insert: Roche AMYL2 reagent. Roche Diagnostic Corp; V10 12/2018)
Supportive Data
In-house studies to verify the cutoff value of 250 U/L showed that 94% (66/70) of pseudocysts had a value of greater than or equal to 250 U/L. Cysts with amylase levels of less than 250 U/L included 69% of adenocarcinomas, 31% of intraductal papillary mucinous neoplasia, 55% of mucinous cystadenomas, 64% serous cystadenomas, and 6% of pseudocysts. Therefore, using a cutoff of less than 250 U/L to exclude a pseudocyst has 94% sensitivity and 42% specificity.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Oncology Test Request (T729)