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HelpTest Code ACLIP Phospholipid (Cardiolipin) Antibodies, IgA, Serum
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Reporting Name
Phospholipid Ab IgA, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimen | Reject |
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Specimen Minimum Volume
0.4 mL
Day(s) Performed
Monday, Thursday
Specimen Retention Time
14 daysReport Available
3 to 5 daysReference Values
APL refers to IgA phospholipid units. One APL unit is 1 microgram of IgA antibody.
Negative: <15.0 APL
Weakly positive: 15.0-39.9 APL
Positive: 40.0-79.9 APL
Strongly positive: ≥80.0 APL
Reference values apply to all ages.
Useful For
May be of diagnostic significance for patients at risk for antiphospholipid syndrome or systemic lupus erythematosus who test negative for criterial antiphospholipid antibodies
CPT Code Information
86147
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ACLIP | Phospholipid Ab IgA, S | 5076-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ACLIP | Phospholipid Ab IgA, S | 5076-5 |
Method Description
Purified cardiolipin antigen is bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells, allowing any cardiolipin antibodies present to bind to the immobilized antigen. Unbound sample is washed away, and an enzyme-labeled antihuman IgA conjugate is added to each well. A second incubation allows the enzyme-labeled antihuman IgA to bind to any patient antibodies that have become attached to the microwells. After washing away any unbound enzyme-labeled antihuman IgA, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. After stopping the enzymatic production of colored product, the presence or absence of cardiolipin antibody is determined by comparing the sample optical density with that of a 5-point calibration curve. Results are reported out semiquantitatively in standard IgA anticardiolipin units.(Package insert: QUANTA Lite ACA IgA III. Inova Diagnostics; Version 22, 08/2020)