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Test Code TCL1A T-Cell Leukemia/Lymphoma Protein 1A (TCL1A) Immunostain, Technical Component Only

Advisory Information

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Additional Information:

1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website:

2. Clients ordering stains using a manual requisition form will not have access to digital images.

3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website:

Useful For

Identification of T-cell leukemia and lymphoma protein overexpression in neoplasms

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

TCL1A IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time
TECHONLY Ambient (preferred)

Reject Due To








Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides

Clinical Information

T-cell leukemia/lymphoma (TCL) proteins augment AKT signal transduction and enhance cell proliferation and survival. Inversion or translocations involving the TCL-1 gene are present in more than 90% of T-cell prolymphocytic leukemias, resulting in overexpression of the TCL-1 protein. TCL-1 is also found in plasmacytoid monocytes in reactive tissues, and the putative malignant counterpart, blastic plasmacytoid dendritic cell neoplasm. In normal tonsil, expression is limited to the B-cell compartment.


This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.


No significant cautionary statements.

Clinical Reference

1.Said JW, Hoyer KK, French SW, et al: TCL1 oncogene expression in B cell subsets from lymphoid hyperplasia and distinct classes of B cell lymphoma. Lab Invest 2001 Apr;81(4):555-564

2. Herling M, Teitell MA, Shen RR, et al: TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood 2003 Jun 15;101(12):5007-5009

Method Description

Immunohistochemistry on sections of paraffin-embedded tissue.(Unpublished Mayo method)

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

1 day

Specimen Retention Time

Until staining is complete.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCL1A TCL1A IHC, Tech Only In Process


Result ID Test Result Name Result LOINC Value
70873 TCL1A IHC, Tech Only No LOINC Needed