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Test Code CAMPY Helicobacter pylori Culture

Performing Laboratory

Mayo Medical Laboratories in Rochester

Reporting Name

Helicobacter pylori Culture

Specimen Type

Varies


Shipping Instructions


Specimen must be received in laboratory within 48 hours of collection.



Necessary Information


Specimen source is required; include the specific anatomic source.



Specimen Required


Preferred:

Specimen Type: Gastric biopsy

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: Acquire biopsied tissue; moisten with sterile saline.

 

Acceptable:

Specimen Type: Gastric brushing or gastric aspirate

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: Aspirate contents with a syringe.


Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Specimen >48 hours old; biopsy submitted in fluid other than sterile saline

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 48 hours

Specimen Minimum Volume

0.5 mL or 0.5 × 0.2 × 0.2-cm sized piece of tissue

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Specimen Retention Time

2 days

Analytic Time

7 days

Reference Values

No growth after 7 days

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
GID Bacteria Identification No, (Bill Only) No
TISSR Tissue Processing No, (Bill Only) No

Useful For

Recovery of Helicobacter pylori from gastric specimens for antimicrobial susceptibility testing of the organism

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

 

See Helicobacter pylori Diagnostic Algorithm in Special Instructions.

Method Name

Conventional Culture Techniques

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87081-Helicobacter pylori culture

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Bacteria identification (if appropriate)

87176-Tissue processing (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CAMPY Helicobacter pylori Culture 587-6

 

Result ID Test Result Name Result LOINC Value
CAMPY Helicobacter pylori Culture 587-6

Clinical Information

Helicobacter pylori is a spiral-shaped gram-negative bacterium that may cause chronic gastritis, peptic ulcer disease, or gastric neoplasia. In adults of industrialized countries, an estimated 0.5% of the susceptible population becomes infected each year, although the incidence has been decreasing over time. The organism may asymptomatically colonize humans. In suspected Helicobacter pylori-associated disease, the noninvasive stool antigen or urea breath test is recommended. If patients fail to respond to treatment and antimicrobial resistance is suspected, a gastric biopsy, gastric brushings, or gastric aspirate may be cultured to attempt to recover the organism for antimicrobial susceptibility testing to assess for resistance.

Interpretation

A positive result provides definitive evidence of the presence of Helicobacter pylori.

 

Organisms may be detected in asymptomatic (colonized) individuals.

 

False-negative culture results may occur since the organism may die between biopsy collection and laboratory culture.

Cautions

Culture-negative results may occur due to the fastidious nature of the organism. Delays in specimen transportation will decrease recovery of the organism. Culture should be set up as soon as possible following specimen collection. Antimicrobial therapy may render the culture negative.

 

Due to Helicobacter pylori's fastidious nature and slow growth, it may take 7 days to recover the organism and up to an additional 21 days to perform antimicrobial susceptibility testing when requested.

Clinical Reference

Theel ES: Helicobacter pylori Infection: Test Utilization Strategies for Diagnosis. Mayo Medical Laboratories Communique 2013;38(6):1-8

Method Description

The selective Helicobacter pylori media used for isolation has a Brucella agar base with added vancomycin, trimethoprim, polymyxin B, and vitamin K1. Fresh medium and high humidity are essential for organism recovery. Plates are incubated at 35° C in a microaerophilic atmosphere.(Lawson AJ: Chapter 57, Helicobacter. In Manual of Clinical Microbiology. 11th edition. Edited by J Jorgensen. ASM Press. Washington DC, 2015, pp 1013-1027)

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)